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anti osteonectin  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti osteonectin
    Anti Osteonectin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+osteonectin/anti-Osteonectin/pmc12753712-48-24-30
    Average 93 stars, based on 20 article reviews
    anti osteonectin - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Adsorption:

    Article Title: Use of Polycaprolactone Electrospun Nanofibers as a Coating for Poly(methyl methacrylate) Bone Cement
    Article Snippet: An OsteoImageTM mineralization assay kit from Lonza was used according to the vendor’s protocol. .. For the protein adsorption test, anti-osteonectin (clone AON-1; Developmental Studies Hybridoma Bank) was used as the primary antibody and goat anti-mouse rhodamine (red) was used as the secondary antibody. ..

    Bioprocessing:

    Article Title: Giant cell tumor of bone. The role of fibroblast growth factor 3 positive mesenchymal stem cells in its pathogenesis.
    Article Snippet: Objectives: Giant cell tumor of bone is typified by massive infiltration of a bland neoplastic stroma by osteoclasts and monocyte progenitors.. The current study aimed at evaluating the nature of the neoplastic cells and the mechanisms underlying the massive giant cell recruitment.. Methods: Five different giant cell tumors were evaluated by immunohistochemistry, and explant cell cultures were established from the same tumors.

    other:

    Article Title: Dynamic assessment of a humanized bone tumour microenvironment reveals insights into osteosarcoma primary tumour remodelling and lung metastases
    Article Snippet: Blocking was performed with 2% BSA in PBS for 10 min at RT before staining in a 1% BSA in PBS solution containing primary antibodies against Osteonectin, Osteopontin (both from DSHB), or Osteocalcin antibody (Abcam) at 4 °C overnight (each 1:100 dilution).

    Immunohistochemistry:

    Article Title: Immunolocalization of Some Epidermal Proteins and Glycoproteins in the Growing Skin of the Australian Lungfish ( Neoceratodus forsteri )
    Article Snippet: .. For immunohistochemistry, the following mouse antibodies were used on tissues of the initial four specimens: anti-osteonectin (AON-1, Termine JDNIDR from NIH, DSHB, Iowa City, IA, USA), anti-osteopontin (MPIIIB10, Solursh M., Franzer A., University of Iowa, DSHB, USA), and anti-alkaline phosphatase (B4–78-s, Katzmann JA, Majo Clinic, Rochester, NY, USA, DSHB, USA). .. For the detection of elastin, a rabbit anti-elastin antibody (ab21610, Abcam, Cambridge, UK) was utilized, and for mucin, a goat anti-mucin antibody (C-20, Santa Cruz Biotech, Santa Cruz, CA, USA).

    Staining:

    Article Title: The immunogenicity and immunomodulatory function of osteogenic cells differentiated from mesenchymal stem cells.
    Article Snippet: .. The slides were stained with primary Abs (anti-rabbit MHC-II (1/1000), anti-osteonectin (ON; Developmental Studies Hybridoma Bank (DSHB); 1/50), anti-osteopontin (OPN; DSHB; 1/50), and anti-osteocalcin (OC; Takara Bio; 1/200)) for 1 h, respectively. .. Subsequently, they were incubated with prediluted Qdot 655 goat F(ab )2 anti-mouse IgG conjugate (H L) (Quantum Dot; 1/200) at 37°C for 1 h. Slides were mounted and observed under confocal microscopy (Olympus FluoView 500).

    Immunopeptidomics:

    Article Title: The immunogenicity and immunomodulatory function of osteogenic cells differentiated from mesenchymal stem cells.
    Article Snippet: .. The slides were stained with primary Abs (anti-rabbit MHC-II (1/1000), anti-osteonectin (ON; Developmental Studies Hybridoma Bank (DSHB); 1/50), anti-osteopontin (OPN; DSHB; 1/50), and anti-osteocalcin (OC; Takara Bio; 1/200)) for 1 h, respectively. .. Subsequently, they were incubated with prediluted Qdot 655 goat F(ab )2 anti-mouse IgG conjugate (H L) (Quantum Dot; 1/200) at 37°C for 1 h. Slides were mounted and observed under confocal microscopy (Olympus FluoView 500).



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    Image Search Results


    MSCs differentiation in cultures over artificial hFGF2-H6 SGs PEA–FN surfaces. (A) Imaging of MSCs by fluorescence microscopy in the presence of artificial hFGF2-H6 SGs at 50 ng/mL for 14 days. Actin, vinculin, vimentin, and YAP were selected as cell markers. Merge refers to actin and vinculin combined fluorescence signals. White squares highlight cell focal adhesions. Close-up pictures of focal adhesions are displayed on the right panels. (B) Fold change on mRNA content (meaning RUNX2, OSN; osteonectin, and OPN; osteopontin gene expression) in MSCs upon incubation with soluble hFGF2-H6 (dark blue), artificial hFGF2-H6 SGs (pale blue), and free Zn 2+ (gray) at 50 ng/mL for 14 days. (C) In-cell Western (ICW) immunodetection of RUNX2, OSN, and OSP proteins in MSCs extracts upon incubation over soluble hFGF2-H6, artificial hFGF2-H6 SGs, and free Zn 2+ (gray) at 50 ng/mL for 14 days. The protein signal is displayed in green, and the cell signal is in red. (D) Statistical analysis of protein signal (green from panel C) expressed as fluorescence per cell and cm 2 in absorbance units). (E) Statistical analysis of cell signal (red from panel C) expressed as fluorescence per cm 2 in absorbance units (au). Peak numbers correspond to the increased percentage of cell growth comparing artificial hFGF2-H6 SGs (pale blue) with soluble hFGF2-H6 (dark blue), free Zn 2+ (gray), and control MSCs (black). Data are expressed as mean ± SEM, and statistical significance is achieved when p < 0.05 is represented as (*). Control refers to MSCs seeded on top of FN-PEA surfaces.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors

    doi: 10.1021/acsami.4c01210

    Figure Lengend Snippet: MSCs differentiation in cultures over artificial hFGF2-H6 SGs PEA–FN surfaces. (A) Imaging of MSCs by fluorescence microscopy in the presence of artificial hFGF2-H6 SGs at 50 ng/mL for 14 days. Actin, vinculin, vimentin, and YAP were selected as cell markers. Merge refers to actin and vinculin combined fluorescence signals. White squares highlight cell focal adhesions. Close-up pictures of focal adhesions are displayed on the right panels. (B) Fold change on mRNA content (meaning RUNX2, OSN; osteonectin, and OPN; osteopontin gene expression) in MSCs upon incubation with soluble hFGF2-H6 (dark blue), artificial hFGF2-H6 SGs (pale blue), and free Zn 2+ (gray) at 50 ng/mL for 14 days. (C) In-cell Western (ICW) immunodetection of RUNX2, OSN, and OSP proteins in MSCs extracts upon incubation over soluble hFGF2-H6, artificial hFGF2-H6 SGs, and free Zn 2+ (gray) at 50 ng/mL for 14 days. The protein signal is displayed in green, and the cell signal is in red. (D) Statistical analysis of protein signal (green from panel C) expressed as fluorescence per cell and cm 2 in absorbance units). (E) Statistical analysis of cell signal (red from panel C) expressed as fluorescence per cm 2 in absorbance units (au). Peak numbers correspond to the increased percentage of cell growth comparing artificial hFGF2-H6 SGs (pale blue) with soluble hFGF2-H6 (dark blue), free Zn 2+ (gray), and control MSCs (black). Data are expressed as mean ± SEM, and statistical significance is achieved when p < 0.05 is represented as (*). Control refers to MSCs seeded on top of FN-PEA surfaces.

    Article Snippet: Cells were incubated with monoclonal primary Abs (1:200) in blocking buffer (PBS/1% milk protein) at room temperature for 2.5 h, respectively; Runx2 (Santa Cruz Biotechnology, C1319), osteonectin (Santa Cruz Biotechnology, SC398419), and osteopontin (Santa Cruz Biotechnology, B1218).

    Techniques: Imaging, Fluorescence, Microscopy, Gene Expression, Incubation, In-Cell ELISA, Immunodetection, Control